vspВопросы современной педиатрииCurrent Pediatrics1682-55271682-5535Издательство «ПедиатрЪ»10.15690/vsp.v21i6.2468vsp-3077Research ArticleОБЗОР ЛИТЕРАТУРЫREVIEWНовые стратегии модификации состава детских молочных смесейNew Strategies for Enhancement of Infant Milk Formulas Compositionhttps://orcid.org/0000-0002-8717-2539БеляеваИ. А.BelyaevaIrina A.

Беляева Ирина Анатольевна, доктор медицинских наук, профессор Российской академии наук, заведующая отделом преконцепционной, антенатальной и неонатальной медицины; профессор кафедры факультетской педиатрии педиатрического факультета; врач-неонатолог.

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irinaneo@mail.ruhttps://orcid.org/0000-0002-6677-2914БомбардироваЕ. П.BombardirovaElena P.

Москва

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https://orcid.org/0000-0002-4955-0121ТуртиТ. В.TurtiTatiana V.

Москва

Moscow

НИИ педиатрии и охраны здоровья детей НКЦ №2 ФГБНУ «РНЦХ им. акад. Б.В. Петровского»; РНИМУ им. Н.И. Пирогова; Морозовская ДГКБРоссияResearch Institute of Pediatrics and Children’s Health in Petrovsky National Research Centre of Surgery; Pirogov Russian National Research Medical University; Morozovskaya Children’s City HospitalRussian FederationНИИ педиатрии и охраны здоровья детей НКЦ №2 ФГБНУ «РНЦХ им. акад. Б.В. Петровского»РоссияResearch Institute of Pediatrics and Children’s Health in Petrovsky National Research Centre of SurgeryRussian FederationНИИ педиатрии и охраны здоровья детей НКЦ №2 ФГБНУ «РНЦХ им. акад. Б.В. Петровского»; РНИМУ им. Н.И. Пирогова; НИИ организации здравоохранения и медицинского менеджментаРоссияResearch Institute of Pediatrics and Children’s Health in Petrovsky National Research Centre of Surgery; Pirogov Russian National Research Medical University; Research Institute for Healthcare Organization and Medical ManagementRussian Federation202230122022216447453Copyright © Беляева И.А., Бомбардирова Е.П., Турти Т.В., 20232023Беляева И.А., Бомбардирова Е.П., Турти Т.В.Belyaeva I.A., Bombardirova E.P., Turti T.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vsp.spr-journal.ru/jour/article/view/3077

В статье обсуждаются проблемы моделирования защитных свойств грудного молока при создании детских молочных смесей. В первую очередь это касается условий формирования нормальной кишечной микробиоты растущего организма, поскольку количественные и качественные ее особенности являются триггером либо саногенетических, либо патологических иммунных и метаболических реакций, а также определяют функционирование оси «кишечник – головной мозг». Показаны протективная значимость разнообразия пребиотического состава молока млекопитающих и индуктивная роль олигосахаридов грудного молока. Представлена современная концепция участия синбиотиков в функционировании желудочно-кишечного тракта и других систем растущего организма, а также использования современных синбиотиков при создании детских молочных смесей, в том числе доступной отечественной смеси.

This article covers the issues of remodeling breast milk’s protective properties during creating infant milk formulas. First of all, this concerns the conditions for normal intestinal microbiota development in growing organism. Its quantitative and qualitative features are the trigger of either sanogenetic, or pathological immune and metabolic reactions, and also determine gut-brain axis functioning. The protective significance of prebiotic composition diversity of mammalian milk and the inductive role of breast milk oligosaccharides are shown. The modern concept of synbiotics role in gastrointestinal tract and other systems functioning, as well as the use of modern synbiotics in the creation of infant formulas (available Russian formula included) are presented.

кишечная микробиотаолигосахаридыпробиотикипребиотикисинбиотикимолочная смесьintestinal microbiotaoligosaccharidesprobioticsprebioticssynbioticsmilk formulaСтатья опубликована при финансовой поддержке АО «ВБД»The article was funded by Wimm Bill DannReferencesVaz JS, Maia MFS, Neves PAR, et al. Monitoring breastfeeding indicators in high-income countries: Levels, trends and challenges. Matern Child Nutr. 2021;17(3):e13137. doi: https://doi.org/10.1111/mcn.13137Vaz JS, Maia MFS, Neves PAR, et al. Monitoring breastfeeding indicators in high-income countries: Levels, trends and challenges. Matern Child Nutr. 2021;17(3):e13137. doi: https://doi.org/10.1111/mcn.13137Neves PAR, Vaz JS, Maia FS, et al. Rates and time trends in the consumption of breastmilk, formula, and animal milk by children younger than 2 years from 2000 to 2019: analysis of 113 countries. Lancet Child Adolesc Health. 2021;5(9):619–630. doi: https://doi.org/10.1016/S2352-4642(21)00163-2Neves PAR, Vaz JS, Maia FS, et al. Rates and time trends in the consumption of breastmilk, formula, and animal milk by children younger than 2 years from 2000 to 2019: analysis of 113 countries. Lancet Child Adolesc Health. 2021;5(9):619–630. doi: https://doi.org/10.1016/S2352-4642(21)00163-2Almeida CC, Mendonca Pereira BF, Leandro KC, et al. Bioactive Compounds in Infant Formula and Their Effects on Infant Nutrition and Health: A Systematic Literature Review. Int J Food Sci. 2021; 2021:8850080. doi: https://doi.org/10.1155/2021/8850080Almeida CC, Mendonca Pereira BF, Leandro KC, et al. Bioactive Compounds in Infant Formula and Their Effects on Infant Nutrition and Health: A Systematic Literature Review. Int J Food Sci. 2021; 2021:8850080. doi: https://doi.org/10.1155/2021/8850080Беляева И.А., Намазова-Баранова Л.С., Бомбардирова Е.П. и др. Таргетное формирование микробиоты младенцев на искусственном вскармливании: современные возможности // Вопросы современной педиатрии. — 2021. — Т. 20. — № 6. — С. 484–491. — doi: https://doi.org/10.15690/vsp.v20i6.2354Belyaeva IA, Namazova-Baranova LS, Bombardirova EP, et al. Targeted Development of Infant Microbiota on Formula Feeding: Modern Options. Voprosy sovremennoi pediatrii — Current Pediatrics. 2021;20(6):484–491. (In Russ). doi: https://doi.org/10.15690/vsp.v20i6.2354Manor O, Dai CL, Kornilov SA, et al. Health and disease markers correlate with gut microbiome composition across thousands of people. Nat Commun. 2020;11(1):5206. doi: https://doi.org/10.1038/s41467-020-18871-1Manor O, Dai CL, Kornilov SA, et al. Health and disease markers correlate with gut microbiome composition across thousands of people. Nat Commun. 2020;11(1):5206. doi: https://doi.org/10.1038/s41467-020-18871-1Heintz-Buschart A, Wilmes P. Human Gut Microbiome: Function Matters. Trends Microbiol. 2018;26(7):563–574. doi: https://doi.org/10.1016/j.tim.2017.11.002Heintz-Buschart A, Wilmes P. Human Gut Microbiome: Function Matters. Trends Microbiol. 2018;26(7):563–574. doi: https://doi.org/10.1016/j.tim.2017.11.002Kelsey CM, Prescott S, McCulloch JA, et al. Gut microbiota composition is associated with newborn functional brain connectivity and behavioral temperament. Brain Behav Immun. 2021;91: 472–486. doi: https://doi.org/10.1016/j.bbi.2020.11.003Kelsey CM, Prescott S, McCulloch JA, et al. Gut microbiota composition is associated with newborn functional brain connectivity and behavioral temperament. Brain Behav Immun. 2021;91: 472–486. doi: https://doi.org/10.1016/j.bbi.2020.11.003Carlson AL, Xia K, Azcarate-Peril MA, et al. Infant Gut Microbiome Associated With Cognitive Development. Biol Psychiatry. 2018;83(2):148–159. doi: https://doi.org/10.1016/j.biopsych.2017.06.021Carlson AL, Xia K, Azcarate-Peril MA, et al. Infant Gut Microbiome Associated With Cognitive Development. Biol Psychiatry. 2018;83(2):148–159. doi: https://doi.org/10.1016/j.biopsych.2017.06.021Indiani CMDSP, Rizzardi KF, Castelo PM, et al. Childhood Obesity and Firmicutes/Bacteroidetes Ratio in the Gut Microbiota: A Systematic Review. Child Obes. 2018;14(8):501–509. doi: https://doi.org/10.1089/chi.2018.0040Indiani CMDSP, Rizzardi KF, Castelo PM, et al. Childhood Obesity and Firmicutes/Bacteroidetes Ratio in the Gut Microbiota: A Systematic Review. Child Obes. 2018;14(8):501–509. doi: https://doi.org/10.1089/chi.2018.0040Lyons KE, Ryan CA, Dempsey EM, et al. Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health. Nutrients. 2020;12(4):1039. doi: https://doi.org/10.3390/nu12041039Lyons KE, Ryan CA, Dempsey EM, et al. Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health. Nutrients. 2020;12(4):1039. doi: https://doi.org/10.3390/nu12041039Hermansson H, Kumar H, Collado MC, et al. Breast Milk Microbiota Is Shaped by Mode of Delivery and Intrapartum Antibiotic Exposure. Front Nutr. 2019;6:4. doi: https://doi.org/10.3389/fnut.2019.00004Hermansson H, Kumar H, Collado MC, et al. Breast Milk Microbiota Is Shaped by Mode of Delivery and Intrapartum Antibiotic Exposure. Front Nutr. 2019;6:4. doi: https://doi.org/10.3389/fnut.2019.00004Chen PW, Lin YL, Huang MS. Profiles of commensal and opportunistic bacteria in human milk from healthy donors in Taiwan. J Food Drug Anal. 2018;26(4):1235–1244. doi: https://doi.org/10.1016/j.jfda.2018.03.004Chen PW, Lin YL, Huang MS. Profiles of commensal and opportunistic bacteria in human milk from healthy donors in Taiwan. J Food Drug Anal. 2018;26(4):1235–1244. doi: https://doi.org/10.1016/j.jfda.2018.03.004Luk B, Veeraragavan S, Engevik M, et al. Postnatal colonization with human “infant-type” Bifidobacterium species alters behavior of adult gnotobiotic mice. PLoS ONE. 2018;13(5):e0196510. doi: https://doi.org/10.1371/journal.pone.0196510Luk B, Veeraragavan S, Engevik M, et al. Postnatal colonization with human “infant-type” Bifidobacterium species alters behavior of adult gnotobiotic mice. PLoS ONE. 2018;13(5):e0196510. doi: https://doi.org/10.1371/journal.pone.0196510Luczynski P, McVey Neufeld KA, Oriach CS, et al. Growing up in a Bubble: Using Germ-Free Animals to Assess the Influence of the Gut Microbiota on Brain and Behavior. Int J Neuropsychopharmacol. 2016;19(8):pyw020. doi: https://doi.org/10.1093/ijnp/pyw020Luczynski P, McVey Neufeld KA, Oriach CS, et al. Growing up in a Bubble: Using Germ-Free Animals to Assess the Influence of the Gut Microbiota on Brain and Behavior. Int J Neuropsychopharmacol. 2016;19(8):pyw020. doi: https://doi.org/10.1093/ijnp/pyw020Desbonnet L, Garrett L, Clarke G, et al. Effects of the probiotic Bifidobacterium infantis in the maternal separation model of depression. Neuroscience. 2010;170(4):1179–1188. doi: https://doi.org/10.1016/j.neuroscience.2010.08.005Desbonnet L, Garrett L, Clarke G, et al. Effects of the probiotic Bifidobacterium infantis in the maternal separation model of depression. Neuroscience. 2010;170(4):1179–1188. doi: https://doi.org/10.1016/j.neuroscience.2010.08.005He X, Slupsky CM, Dekker JW, et al. Integrated Role of Bifidobacterium animalis subsp. lactis Supplementation in Gut Microbiota, Immunity, and Metabolism of Infant Rhesus Monkeys. mSystems. 2016;1(6):e00128-16. doi: https://doi.org/10.1128/mSystems.00128-16He X, Slupsky CM, Dekker JW, et al. Integrated Role of Bifidobacterium animalis subsp. lactis Supplementation in Gut Microbiota, Immunity, and Metabolism of Infant Rhesus Monkeys. mSystems. 2016;1(6):e00128-16. doi: https://doi.org/10.1128/mSystems.00128-16Abelius MS, Ernerudh J, Berg G, et al. High cord blood levels of the T-helper 2-associated chemokines CCL17 and CCL22 precede allergy development during the first 6 years of life. Pediatr Res. 2011;70(5):495–500. doi: https://doi.org/10.1203/PDR.0b013e31822f2411Abelius MS, Ernerudh J, Berg G, et al. High cord blood levels of the T-helper 2-associated chemokines CCL17 and CCL22 precede allergy development during the first 6 years of life. Pediatr Res. 2011;70(5):495–500. doi: https://doi.org/10.1203/PDR.0b013e31822f2411Sanders ME. Summary of probiotic activities of Bifidobacterium lactis HN019. J Clin Gastroenterol. 2006;40(9):776–783. doi: http://doi.org/10.1097/01.mcg.0000225576.73385.f0Sanders ME. Summary of probiotic activities of Bifidobacterium lactis HN019. J Clin Gastroenterol. 2006;40(9):776–783. doi: http://doi.org/10.1097/01.mcg.0000225576.73385.f0Zhou JS, Shu Q, Rutherfurd KJ, et al. Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria. Food Chem Toxicol. 2000;38(2-3):153–161. doi: http://doi.org/10.1016/S0278-6915(99)00154-4Zhou JS, Shu Q, Rutherfurd KJ, et al. Acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria. Food Chem Toxicol. 2000;38(2-3):153–161. doi: http://doi.org/10.1016/S0278-6915(99)00154-4Neis EPJG, Dejong CHC, Rensen SS. The role of microbial amino acid metabolism in host metabolism. Nutrients. 2015;7(4): 2930–2946. doi: http://doi.org/10.3390/nu7042930Neis EPJG, Dejong CHC, Rensen SS. The role of microbial amino acid metabolism in host metabolism. Nutrients. 2015;7(4): 2930–2946. doi: http://doi.org/10.3390/nu7042930Martin FP, Sprenger N, Yap IK, et al. Panorganismal gut microbiome-host metabolic crosstalk. J Proteome Res. 2009; 8(4):2090–2105. doi: http://doi.org/10.1021/pr801068xMartin FP, Sprenger N, Yap IK, et al. Panorganismal gut microbiome-host metabolic crosstalk. J Proteome Res. 2009; 8(4):2090–2105. doi: http://doi.org/10.1021/pr801068xCollado MC, Cernada M, Baüerl C, et al. Microbial ecology and host-microbiota interactions during early life stages. Gut Microbes. 2012;3(4):352–365. doi: https://doi.org/10.4161/gmic.21215Collado MC, Cernada M, Baüerl C, et al. Microbial ecology and host-microbiota interactions during early life stages. Gut Microbes. 2012;3(4):352–365. doi: https://doi.org/10.4161/gmic.21215Marcobal A, Sonnenburg JL. Human milk oligosaccharide consumption by intestinal microbiota. Clin Microbiol Infect. 2012;18 Suppl 4(0 4):12–15. doi: https://doi.org/10.1111/j.1469-0691.2012.03863.xMarcobal A, Sonnenburg JL. Human milk oligosaccharide consumption by intestinal microbiota. Clin Microbiol Infect. 2012;18 Suppl 4(0 4):12–15. doi: https://doi.org/10.1111/j.1469-0691.2012.03863.xCarrothers JM, York MA, Brooker SL, et al. Fecal Microbial Community Structure Is Stable over Time and Related to Variation in Macronutrient and Micronutrient Intakes in Lactating Women. J Nutr. 2015;145(10):2379–2388. doi: https://doi.org/10.3945/jn.115.211110Carrothers JM, York MA, Brooker SL, et al. Fecal Microbial Community Structure Is Stable over Time and Related to Variation in Macronutrient and Micronutrient Intakes in Lactating Women. J Nutr. 2015;145(10):2379–2388. doi: https://doi.org/10.3945/jn.115.211110Cheema AS, Trevenen ML, Turlach BA, et al. Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes. Int J Mol Sci. 2022;23(5):2804. doi: https://doi.org/10.3390/ijms23052804Cheema AS, Trevenen ML, Turlach BA, et al. Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes. Int J Mol Sci. 2022;23(5):2804. doi: https://doi.org/10.3390/ijms23052804Asnicar F, Manara S, Zolfo M, et al. Studying vertical microbiome transmission from mothers to infants by strain-level metagenomic profiling. mSystems. 2017;2(1):e00164-16. doi: https://doi.org/10.1128/mSystems.00164-16Asnicar F, Manara S, Zolfo M, et al. Studying vertical microbiome transmission from mothers to infants by strain-level metagenomic profiling. mSystems. 2017;2(1):e00164-16. doi: https://doi.org/10.1128/mSystems.00164-16Duranti S, Lugli GA, Mancabelli L, et al. Maternal inheritance of bifidobacterial communities and bifidophages in infants through vertical transmission. Microbiome. 2017;5(1):66. doi: https://doi.org/10.1186/s40168-017-0282-6Duranti S, Lugli GA, Mancabelli L, et al. Maternal inheritance of bifidobacterial communities and bifidophages in infants through vertical transmission. Microbiome. 2017;5(1):66. doi: https://doi.org/10.1186/s40168-017-0282-6Laursen MF, Sakanaka M, von Burg N, et al. Bifidobacterium species associated with breastfeeding produce aromatic lactic acids in the infant gut. Nat Microbiol. 2021;6(11):1367–1382. doi: https://doi.org/10.1038/s41564-021-00970-4Laursen MF, Sakanaka M, von Burg N, et al. Bifidobacterium species associated with breastfeeding produce aromatic lactic acids in the infant gut. Nat Microbiol. 2021;6(11):1367–1382. doi: https://doi.org/10.1038/s41564-021-00970-4Ehrlich AM, Pacheco AR, Henrick BM, et al. Indole-3-lactic acid associated with Bifidobacterium-dominated microbiota significantly decreases inflammation in intestinal epithelial cells. BMC Microbiol. 2020;20(1):357. doi: https://doi.org/10.1186/s12866-020-02023-yEhrlich AM, Pacheco AR, Henrick BM, et al. Indole-3-lactic acid associated with Bifidobacterium-dominated microbiota significantly decreases inflammation in intestinal epithelial cells. BMC Microbiol. 2020;20(1):357. doi: https://doi.org/10.1186/s12866-020-02023-yMarcobal A, Barboza M, Froehlich JW, et al. Consumption of human milk oligosaccharides by gut-related microbes. J Agric Food Chem. 2010;58(9):5334–5340. doi: https://doi.org/10.1021/jf9044205Marcobal A, Barboza M, Froehlich JW, et al. Consumption of human milk oligosaccharides by gut-related microbes. J Agric Food Chem. 2010;58(9):5334–5340. doi: https://doi.org/10.1021/jf9044205Zambruni M, Villalobos A, Somasunderam A, et al. Maternal and pregnancy-related factors affecting human milk cytokines among Peruvian mothers bearing low-birth-weight neonates. J Reprod Immunol. 2017;120:20–26. doi: https://doi.org/10.1016/j.jri.2017.04.001Zambruni M, Villalobos A, Somasunderam A, et al. Maternal and pregnancy-related factors affecting human milk cytokines among Peruvian mothers bearing low-birth-weight neonates. J Reprod Immunol. 2017;120:20–26. doi: https://doi.org/10.1016/j.jri.2017.04.001Vass RA, Kemeny A, Dergez T, et al. Distribution of bioactive factors in human milk samples. Int Breastfeed J. 2019;14:9. doi: https://doi.org/10.1186/s13006-019-0203-3Vass RA, Kemeny A, Dergez T, et al. Distribution of bioactive factors in human milk samples. Int Breastfeed J. 2019;14:9. doi: https://doi.org/10.1186/s13006-019-0203-3Hennet T, Borsig L. Breastfed at Tiffany’s. Trends Biochem Sci. 2016;41(6):508–518. doi: https://doi.org/10.1016/j.tibs.2016.02.008Hennet T, Borsig L. Breastfed at Tiffany’s. Trends Biochem Sci. 2016;41(6):508–518. doi: https://doi.org/10.1016/j.tibs.2016.02.008Le Doare K, Holder B, Bassett A, Pannaraj PS. Mother’s Milk: A Purposeful Contribution to the Development of the Infant Microbiota and Immunity. Front Immunol. 2018;9:361. doi: https://doi.org/10.3389/fimmu.2018.00361Le Doare K, Holder B, Bassett A, Pannaraj PS. Mother’s Milk: A Purposeful Contribution to the Development of the Infant Microbiota and Immunity. Front Immunol. 2018;9:361. doi: https://doi.org/10.3389/fimmu.2018.00361Davis EC, Wang M, Donovan SM. The role of early life nutrition in the establishment of gastrointestinal microbial composition and function. Gut Microbes. 2017;8(2):143–171. doi: https://doi.org/10.1080/19490976.2016.1278104Davis EC, Wang M, Donovan SM. The role of early life nutrition in the establishment of gastrointestinal microbial composition and function. Gut Microbes. 2017;8(2):143–171. doi: https://doi.org/10.1080/19490976.2016.1278104Thurl S, Munzert M, Boehm G, et al. Systematic review of the concentrations of oligosaccharides in human milk. Nutr Rev. 2017; 75(11):920–933. doi: https://doi.org/10.1093/nutrit/nux044Thurl S, Munzert M, Boehm G, et al. Systematic review of the concentrations of oligosaccharides in human milk. Nutr Rev. 2017; 75(11):920–933. doi: https://doi.org/10.1093/nutrit/nux044Quinn EM, Joshi L, Hickey RM. Symposium review: Dairy-derived oligosaccharides-Their influence on host-microbe interactions in the gastrointestinal tract of infants. J Dairy Sci. 2020;103(4): 3816–3827. doi: https://doi.org/10.3168/jds.2019-17645Quinn EM, Joshi L, Hickey RM. Symposium review: Dairy-derived oligosaccharides-Their influence on host-microbe interactions in the gastrointestinal tract of infants. J Dairy Sci. 2020;103(4): 3816–3827. doi: https://doi.org/10.3168/jds.2019-17645Coppa GV, Gabrielli O, Zampini L, et al. Oligosaccharides in 4 different milk groups, Bifidobacteria, and Ruminococcus obeum. J Pediatr Gastroenterol Nutr. 2011;53(1):80–87. doi: https://doi.org/10.1097/MPG.0b013e3182073103Coppa GV, Gabrielli O, Zampini L, et al. Oligosaccharides in 4 different milk groups, Bifidobacteria, and Ruminococcus obeum. J Pediatr Gastroenterol Nutr. 2011;53(1):80–87. doi: https://doi.org/10.1097/MPG.0b013e3182073103Goehring KC, Kennedy AD, Prieto PA, et al. Direct evidence for the presence of human milk oligosaccharides in the circulation of breastfed infants. PLoS One. 2014;9:e101692 doi: https://doi.org/10.1371/journal.pone.0101692Goehring KC, Kennedy AD, Prieto PA, et al. Direct evidence for the presence of human milk oligosaccharides in the circulation of breastfed infants. PLoS One. 2014;9:e101692 doi: https://doi.org/10.1371/journal.pone.0101692Soyyılmaz B, Mikš MH, Röhrig CH, et al. The Mean of Milk: A Review of Human Milk Oligosaccharide Concentrations throughout Lactation. Nutrients. 2021;13(8):2737. doi: https://doi.org/10.3390/nu13082737Soyyılmaz B, Mikš MH, Röhrig CH, et al. The Mean of Milk: A Review of Human Milk Oligosaccharide Concentrations throughout Lactation. Nutrients. 2021;13(8):2737. doi: https://doi.org/10.3390/nu13082737Wiciński M, Sawicka E, Gębalski J, et al. Human Milk Oligosaccharides: Health Benefits, Potential Applications in Infant Formulas, and Pharmacology. Nutrients. 2020;12(1):266. doi: https://doi.org/10.3390/nu12010266Wiciński M, Sawicka E, Gębalski J, et al. Human Milk Oligosaccharides: Health Benefits, Potential Applications in Infant Formulas, and Pharmacology. Nutrients. 2020;12(1):266. doi: https://doi.org/10.3390/nu12010266Lin AE, Autran CA, Szyszka A, et al. Human milk oligosaccharides inhibit growth of group B. Streptococcus. J Biol Chem. 2017;292(27):11243–11249. doi: https://doi.org/10.1074/jbc.M117.789974Lin AE, Autran CA, Szyszka A, et al. Human milk oligosaccharides inhibit growth of group B. Streptococcus. J Biol Chem. 2017;292(27):11243–11249. doi: https://doi.org/10.1074/jbc.M117.789974Donovan SM, Comstock SS. Human Milk Oligosaccharides Influence Neonatal Mucosal and Systemic Immunity. Ann Nutr Metab. 2016;69(Suppl 2):42–51. doi: https://doi.org/10.1159/000452818Donovan SM, Comstock SS. Human Milk Oligosaccharides Influence Neonatal Mucosal and Systemic Immunity. Ann Nutr Metab. 2016;69(Suppl 2):42–51. doi: https://doi.org/10.1159/000452818Steenhout P, Sperisen P, Martin FP, et al. Term infant formula supplemented with human milk oligosaccharides (2' fucosyllactose and lacto-N-neotetraose) shifts stool microbiota and metabolic signatures closer to that of breastfed infants. J Pediatr Gastroenterol Nutr. 2016;63(Suppl 1):S55.Steenhout P, Sperisen P, Martin FP, et al. Term infant formula supplemented with human milk oligosaccharides (2' fucosyllactose and lacto-N-neotetraose) shifts stool microbiota and metabolic signatures closer to that of breastfed infants. J Pediatr Gastroenterol Nutr. 2016;63(Suppl 1):S55.Chichlowski M, German JB, Lebrilla CB, Mills DA. The Influence of Milk Oligosaccharides on Microbiota of Infants: Opportunities for Formulas. Annu Rev Food Sci Technol. 2001;2:331–351. doi: https://doi.org/10.1146/annurev-food-022510-133743Chichlowski M, German JB, Lebrilla CB, Mills DA. The Influence of Milk Oligosaccharides on Microbiota of Infants: Opportunities for Formulas. Annu Rev Food Sci Technol. 2001;2:331–351. doi: https://doi.org/10.1146/annurev-food-022510-133743Vandenplas Y, Żołnowska M, Berni Canani R, et al. Effects of an Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides on Growth, Tolerability, Safety and Infection Risk in Infants with Cow’s Milk Protein Allergy: A Randomized, Multi-Center Trial. Nutrients. 2022;14(3):530. doi: https://doi.org/10.3390/nu14030530Vandenplas Y, Żołnowska M, Berni Canani R, et al. Effects of an Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides on Growth, Tolerability, Safety and Infection Risk in Infants with Cow’s Milk Protein Allergy: A Randomized, Multi-Center Trial. Nutrients. 2022;14(3):530. doi: https://doi.org/10.3390/nu14030530GRAS Exemption Claim for 2'-O-Fucosyllactose (2'-FL). GRAS Notice (GRN) No. 650. In: U.S. Food & Drug Administration. Available online: https://www.fda.gov/media/99125/download. Accessed on December 06, 2022.GRAS Exemption Claim for 2'-O-Fucosyllactose (2'-FL). GRAS Notice (GRN) No. 650. In: U.S. Food & Drug Administration. Available online: https://www.fda.gov/media/99125/download. Accessed on December 06, 2022.GRAS Exemption Claim for Lacto-N-neotetraose(LNnT). GRAS Notice (GRN) No. 659. In: U.S. Food & Drug Administration. Available online: https://www.fda.gov/media/100020/download. Accessed on December 06, 2022.GRAS Exemption Claim for Lacto-N-neotetraose(LNnT). GRAS Notice (GRN) No. 659. In: U.S. Food & Drug Administration. Available online: https://www.fda.gov/media/100020/download. Accessed on December 06, 2022.Salminen S, Stahl B, Vinderola G, Szajewska H. Infant Formula Supplemented with Biotics: Current Knowledge and Future Perspectives. Nutrients. 2020;12(7):1952. doi: https://doi.org/10.3390/nu12071952Salminen S, Stahl B, Vinderola G, Szajewska H. Infant Formula Supplemented with Biotics: Current Knowledge and Future Perspectives. Nutrients. 2020;12(7):1952. doi: https://doi.org/10.3390/nu12071952Hill C, Guarner F, Reid G, et al. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11(8):506–514. doi: https://doi.org/10.1038/nrgastro.2014.66Hill C, Guarner F, Reid G, et al. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014;11(8):506–514. doi: https://doi.org/10.1038/nrgastro.2014.66Swanson KS, Gibson GR, Hutkins R, et al. The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics. Nat Rev Gastroenterol Hepatol. 2020;17(11):687–701. doi: https://doi.org/10.1038/s41575-020-0344-2Swanson KS, Gibson GR, Hutkins R, et al. The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of synbiotics. Nat Rev Gastroenterol Hepatol. 2020;17(11):687–701. doi: https://doi.org/10.1038/s41575-020-0344-2Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr. 1995;125(6):1401–1412. doi: https://doi.org/10.1093/jn/125.6.1401Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr. 1995;125(6):1401–1412. doi: https://doi.org/10.1093/jn/125.6.1401Srivastava A, Mishra S. Enrichment and evaluation of galactooligosaccharides produced by whole cell treatment of sugar reaction mixture. Mol Biol Rep. 2019;46(1):1181–1188. doi: https://doi.org/10.1007/s11033-019-04585-1Srivastava A, Mishra S. Enrichment and evaluation of galactooligosaccharides produced by whole cell treatment of sugar reaction mixture. Mol Biol Rep. 2019;46(1):1181–1188. doi: https://doi.org/10.1007/s11033-019-04585-1Porras-Dominguez JR, Rodríguez-Alegría ME, Miranda A, et al. Frucooligosaccharides purification: complexing simple sugars with phenylboronic acid. Food Chem. 2019;285:204–212. doi: https://doi.org/10.1016/j.foodchem.2019.01.130Porras-Dominguez JR, Rodríguez-Alegría ME, Miranda A, et al. Frucooligosaccharides purification: complexing simple sugars with phenylboronic acid. Food Chem. 2019;285:204–212. doi: https://doi.org/10.1016/j.foodchem.2019.01.130Childs CE. Röytiö H, Alhoniemi E, et al. Xylo-oligosaccharides alone or in synbiotic combination with Bifidobacterium animalis subsp. lactis induce bifidogenesis and modulate markers of immune function in healthy adults: a double-blind, placebo-controlled, randomised, factorial cross-over study. Br J Nutr. 2014;111: 1945–1956. doi: https://doi.org/10.1017/S0007114513004261Childs CE. Röytiö H, Alhoniemi E, et al. Xylo-oligosaccharides alone or in synbiotic combination with Bifidobacterium animalis subsp. lactis induce bifidogenesis and modulate markers of immune function in healthy adults: a double-blind, placebo-controlled, randomised, factorial cross-over study. Br J Nutr. 2014;111: 1945–1956. doi: https://doi.org/10.1017/S0007114513004261Favretto DC, Pontin B, Moreira TR. Effect of the consumption of a cheese enriched with probiotic organisms (Bifidobacterium lactis Bi-07) in improving symptoms of constipation. Arq Gastroenterol. 2013;50(3):196–201. doi: https://doi.org/10.1590/S0004-28032013000200035Favretto DC, Pontin B, Moreira TR. Effect of the consumption of a cheese enriched with probiotic organisms (Bifidobacterium lactis Bi-07) in improving symptoms of constipation. Arq Gastroenterol. 2013;50(3):196–201. doi: https://doi.org/10.1590/S0004-28032013000200035Krumbeck JA, Rasmussen HE, Hutkins RW, et al. Probiotic Bifidobacterium strains and galactooligosaccharides improve intestinal barrier function in obese adults but show no synergism when used together as synbiotics. Microbiome. 2018;6(1):121. doi: https://doi.org/10.1186/s40168-018-0494-4Krumbeck JA, Rasmussen HE, Hutkins RW, et al. Probiotic Bifidobacterium strains and galactooligosaccharides improve intestinal barrier function in obese adults but show no synergism when used together as synbiotics. Microbiome. 2018;6(1):121. doi: https://doi.org/10.1186/s40168-018-0494-4Украинцев С.Е., Парамонова Н.С., Малёванная И.А. Грудное молоко: возможные механизмы формирования поведения и когнитивных функций ребенка // Вопросы современной педиатрии. — 2018. — Т. 17. — № 5. — С. 394–398. — doi: https://doi.org/10.15690/vsp.v17i5.1956Ukraintsev SЕ, Paramonova NS, Malyovannaya IА. Breast Milk: Possible Mechanisms to Shape the Behaviour and Cognitive Functions of a Child. Voprosy sovremennoi pediatrii — Current Pediatrics. 2018;7(5):394–398. (In Russ). doi: https://doi.org/10.15690/vsp.v17i5.1956Vandenplas Y, Analitis A, Tziouvara C, et al. Safety of a New Synbiotic Starter Formula. Pediatr Gastroenterol Hepatol Nutr. 2017;20(3): 167–177. doi: https://doi.org/10.5223/pghn.2017.20.3.167Vandenplas Y, Analitis A, Tziouvara C, et al. Safety of a New Synbiotic Starter Formula. Pediatr Gastroenterol Hepatol Nutr. 2017;20(3): 167–177. doi: https://doi.org/10.5223/pghn.2017.20.3.167Bocquet A, Lachambre E, Kempf C, Beck L. Effect of infant and follow-on formulas containing B lactis and galacto- and fructooligosaccharides on infection in healthy term infants. J Pediatr Gastroenterol Nutr. 2013;57(2):180–187. doi: https://doi.org/10.1097/MPG.0b013e318297f35eBocquet A, Lachambre E, Kempf C, Beck L. Effect of infant and follow-on formulas containing B lactis and galacto- and fructooligosaccharides on infection in healthy term infants. J Pediatr Gastroenterol Nutr. 2013;57(2):180–187. doi: https://doi.org/10.1097/MPG.0b013e318297f35eBraegger C, Chmielewska A, Decsi T, et al. Supplementation of infant formula with probiotics and/or prebiotics: a systematic review and comment by the ESPGHAN committee on nutrition. J Pediatr Gastroenterol Nutr. 2011;52(2):238–250. doi: https://doi.org/10.1097/MPG.0b013e3181fb9e80Braegger C, Chmielewska A, Decsi T, et al. Supplementation of infant formula with probiotics and/or prebiotics: a systematic review and comment by the ESPGHAN committee on nutrition. J Pediatr Gastroenterol Nutr. 2011;52(2):238–250. doi: https://doi.org/10.1097/MPG.0b013e3181fb9e80Holscher HD, Czerkies LA, Cekola P, et al. Bifidobacterium lactis Bb12 enhances intestinal antibody response in formula-fed infants: a randomized, double-blind, controlled trial. JPEN J Parenter Enteral Nutr. 2012;36(1 Suppl):106S–117S. doi: https://doi.org/10.1177/0148607111430817Holscher HD, Czerkies LA, Cekola P, et al. Bifidobacterium lactis Bb12 enhances intestinal antibody response in formula-fed infants: a randomized, double-blind, controlled trial. JPEN J Parenter Enteral Nutr. 2012;36(1 Suppl):106S–117S. doi: https://doi.org/10.1177/0148607111430817Vandenplas Y, Abkari A, Bellaiche M, et al. Prevalence and health outcomes of functional gastrointestinal symptoms in infants from birth to 12 months of age. J Pediatr Gastroenterol Nutr. 2015;61(5):531–537. doi: https://doi.org/10.1097/MPG.0000000000000949Vandenplas Y, Abkari A, Bellaiche M, et al. Prevalence and health outcomes of functional gastrointestinal symptoms in infants from birth to 12 months of age. J Pediatr Gastroenterol Nutr. 2015;61(5):531–537. doi: https://doi.org/10.1097/MPG.0000000000000949

The authors declare that there are no conflicts of interest present.