INFLUENCE OF THE MILK FORMULA ENRICHED WITH BIFIDOBACTERIA STRAIN BB12 ON DEVELOPMENT OF INTESTINAL MICROFLORA IN NEWBORNS
https://doi.org/10.15690/vsp.v11i4.358
Abstract
The study deals with qualitative and quantitative structure of normal intestinal microflora in newborns before and after feeding with the formula «Agusha Gold 1» containing the strain B. lactis BB12. The specific structure of the intestinal bifidobacreria is studied in detail by means of the real-time polymerase chain reaction. It was demonstrated, that during the feeding with this formula there was a tendency towards development of the microbiota and specific structure of the intestinal bifidobacteria similar to those of the breast-fed children.
About the Authors
E. V. Kulagina
N.I. Pirogov Russian National Research Medical University, Moscow
Russian Federation
Russian Federation
Z. A. Chernaya
N.I. Pirogov Russian National Research Medical University, Moscow
Russian Federation
Russian Federation
A. N. Shkoporov
N.I. Pirogov Russian National Research Medical University, Moscow
Russian Federation
Russian Federation
E. V. Khokhlova
N.I. Pirogov Russian National Research Medical University, Moscow
Russian Federation
Russian Federation
B. V. Efimov
N.I. Pirogov Russian National Research Medical University, Moscow
Russian Federation
Russian Federation
Yu. M. Golubtsova
N.I. Pirogov Russian National Research Medical University, Moscow
Russian Federation
Russian Federation
L. I. Kafarskaya
N.I. Pirogov Russian National Research Medical University, Moscow
Russian Federation
Lyudmila Kafarskaya, Doctor of Medical Science, professor, head of microbiology and virology chair
Russian Federation
Lyudmila Kafarskaya, Doctor of Medical Science, professor, head of microbiology and virology chair
References
1. Harmsen H. J., Wildeboer-Veloo A. C., Raangs G. C. et al. Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods. J. Pediatr Gastroenterol Nutr. 2000; 30 (1): 61–67.
2. Satokari R.M., Vaughan E.E., Smidt H. et al. Molecular approaches for the detection and identification of bifidobacteria and lactobacilli in the human gastrointestinal tract. Syst Appl Microbiol. 2003; 26 (4): 572–584.
3. Howie P. W., Forsyth J. S., Ogston S. A. et al. Protective effect of breast feeding against infection. BMJ. 1990; 300 (6716): 11–16.
4. van Odijk J., Kull I., Borres M. P. et al. Breastfeeding and allergic disease: a multidisciplinary review of the literature (1966–2001) on the mode of early feeding in infancy and its impact on later atopic manifestations. Allergy. 2003; 58 (9): 833–843.
5. Edwards C.A., Parrett A. M. Intestinal flora during the first months of life: new perspectives. Br. J. Nutr. 2002; 88 (Suppl. 1): S11–18.
6. Junick J., Blaut M. Quantification of human fecal Bifidobacterium species by quantitative real-time PCR targeting the groEL gene. Appl. Environ. Microbiol. 2012.
7. Salminen S., Benno Y., de Vos W. Intestinal colonisation, microbiota and future probiotics? Asia Pac. J. Clin. Nutr. 2006; 15 (4): 558–562.
8. Matsuki T., Watanabe K., Fujimoto J. et al. Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces. Appl. Environ. Microbiol. 2002; 68 (11): 5445–5451.
9. Matsuki T., Watanabe K., Fujimoto J. et al. Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces. Appl. Environ. Microbiol. 2004; 70 (12): 7220–7228.
10. Коршунов В. М., Смеянов В. В., Ефимов Б. А. Рациональные подходы к проблеме коррекции микрофлоры кишечника. Вестник Российской академии медицинских наук. 1996; 2: 60–64.
11. Backhed F., Ley R. E. et al. Host-bacterial mutualism in the human intestine. Science. 2005; 307 (5717): 1915–1920.
12. Бондаренко В. М., Воробьев А. А. Дисбиозы и перараты с про- биотической функцией. Журн. микробиол. эпидемиол. иммуно- биол. 2004; 1: 84–92.
13. Eckburg P. B., Bik E. M., Bernstein C. N. et al. Divercity of the human intestinal microbial flora. Science. 2005; 308: 1635–1638.
14. Ефимов Б. А. Микроэкология кишечника человека, коррекция микрофлоры при дисбиотических состояниях. Дис. … докт. мед. наук. Москва. 2005.
15. Park H. K., Shim S. S., Kim S. Y. et al. Molecular analysis of colonized bacteria in a human newborn infant gut. J. Microbiol. 2005; 43 (4): 345–353.
16. Matsuki T., Watanabe K., Tanaka R. et al. Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers. Appl. Environ. Microbiol. 1999; 65 (10): 4506–4512.
17. Mackie R. I., Sghir A., Gaskins H. R. Developmental microbial ecology of the neonatal gastrointestinal tract. Am. J. Clin. Nutr. 1999; 69 (5): 1035S–1045S.
18. Morelli L. Postnatal development of intestinal microflora as influenced by infant nutrition. J. Nutr. 2008; 138 (9): 1791–1795.
Review
For citations:
Kulagina E.V., Chernaya Z.A., Shkoporov A.N., Khokhlova E.V., Efimov B.V., Golubtsova Yu.M., Kafarskaya L.I. INFLUENCE OF THE MILK FORMULA ENRICHED WITH BIFIDOBACTERIA STRAIN BB12 ON DEVELOPMENT OF INTESTINAL MICROFLORA IN NEWBORNS. Current Pediatrics. 2012;11(4):47-53. (In Russ.) https://doi.org/10.15690/vsp.v11i4.358
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