MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION
https://doi.org/10.15690/vsp.v12i6.871
Abstract
Aim: to compare two methods of S. pneumoniae molecular typing: classic serological method and the multiplex polymerase chain reaction (M-PCR) assay modified in accordance to the date on the serotypes circulating in Russian Federation. Patients and methods: 420 pneumococcal isolates mainly from non-sterile loci were analyzed. After microbiological identification pneumococci were serologically typed with the means of specific antiserum produced by Staten Serum Institute (Denmark) in latex agglutination test and/or capsular swelling method. At the same time we performed series of the M-PCR, which consisted of 7 consecutive reactions at the most. Results: serotype was identified by the means of serological method in all 420 strains of S. pneumoniae; in total we determined 24 different serotypes. By the means of the M-PCR we succeeded in identification of 95% (399/420) examined strains, and 90% of them were typed during the first 3 reactions. All isolates failed to be typed by M-PCR (n =21) have serotypes not included into the composition of the M-PCR. The results of serological and molecular typing were identical in 99,2% (396/399) of the isolates; 3 strains showed contradictory results: serotype 19A was found on serological assay and serotype 19F — on PCR assay. Conclusions: the introduced modification of M-PCR allows correct identification of pneumococcal serotype more than in 90% of strains circulating in the Russian Federation, including all serotypes of pneumococcal polysaccharide conjugate vaccine.
About the Authors
N. M. Alyab'eva
Scientific Centre of Children Health of RAMS, Moscow
Russian Federation
Alyabyeva Natalya Mikhaylovna, Junior Researcher at the Laboratory of Experimental Immunology and Virology
Russian Federation
Alyabyeva Natalya Mikhaylovna, Junior Researcher at the Laboratory of Experimental Immunology and Virology
T. A. Blinova
Scientific Centre of Children Health of RAMS, Moscow
Russian Federation
Russian Federation
O. A. Ponomarenko
Scientific Centre of Children Health of RAMS, Moscow
Russian Federation
Russian Federation
A. V. Lazareva
Scientific Centre of Children Health of RAMS, Moscow
Russian Federation
Russian Federation
L. K. Katosova
Scientific Centre of Children Health of RAMS, Moscow
Russian Federation
Russian Federation
N. A. Mayanskii
Scientific Centre of Children Health of RAMS, Moscow;
I.M. Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
References
1. . Reinert R. R., Paradiso P., Fritzel B. Advances in pneumococcal vaccines: the 13-valent pneumococcal conjugate vaccine received market authorization in Europe. Exp. Rev. Vac. 2010; 9: 229–236.
2. L.K. Katosova, T.V. Spichak, S.S. Kim, S.B. Yatsyishina, I.V. Zubkova, M.N. Praded. Issues of Pediatric Diagnosis. 2009; 2: 27-31.
3. S.V. Sidorenko, Y.V. Lobzin, S.M. Kharit, I.S. Koroleva, V.K. Tatochenko. Pneumococcal infection and the modern means of preventing thereof – epidemiological review of the situation in Russia and around the world. Issues of Modern Pediatrics. 2010; 9 (1): 62-69.
4. McIntosh E. D., Reinert R. R. Global prevailing and emerging pediatric pneumococcal serotypes. Exp. Rev Vac. 2011; 10(1): 109–129. doi: 10.1586/erv.10.145.
5. R.R. Raynert, B. Tayshi. New data on efficacy of the 13-valent pneumococcal conjugate vaccine against invasive pneumococcal infections, pneumonias, acute otitis media and nasopharyngeal carriage. Pediatric Pharmacology. 2012; 3: 12-18.
6. Rodgers G. L., Arguedas A., Cohen R., Dagan R. Global serotype distribution among S. pneumoniae isolates causing otitis media in children: potential implications for pneumococcal conjugate vaccines. Vaccine. 2009; 27: 3802–3810.
7. Rodgers G. L., Arguedas A., Cohen R., Dagan R. Global serotype distribution among S. pneumoniae isolates causing otitis media in children: potential implications for pneumococcal conjugate vaccines. Vaccine. 2009; 27: 3802–3810.
8. A.N. Mayanskiy. Streptococcus: microbiology and pathology. Issues of Pediatric Diagnosis. 2010; 1: 9-19.
9. L.K. Katosova, V.K. Tatochenko, A.A. Arova, A.A. Keshikbaeva, A.P. Baturo, T.A. Kuznetsova, A.B. Levin. Streptococcus pneumoniae serotypes in children with acute pneumonia or pleuritic. Journal of Microbiology, Epidemiology and Immunobiology. 1990; 5: 23-28.
10. N.A. Mayanskiy, N.M. Alyab’eva, L.K. Katosova, T.A. Grechukha, V.G. Pinelis, L.S. Namazova-Baranova. Identification of capsule pneumococcal serotypes using multiplex PCR assay. Issues of Pediatric Diagnosis. 2010; 2(6): 6-10.
11. Techasaensiri C., Messina A. F., Katz K., Ahmad N., Huang R., McCracken G. H., Jr. Epidemiology and evolution of invasive pneumococcal disease caused by multidrug resistant serotypes of 19A in the 8 years after implementation of pneumococcal conjugate vaccine immunization in Dallas, Texas. Pediatr. Infect. Dis. J. 2010; 29 (4): 294–300.
12. Miller E., Andrews N. J., Waight P. A., Slack M. P., George R. C. Herd immunity and serotype replacement 4 years after sevenvalent pneumococcal conjugate vaccination in England and Wales: an observational cohort study. Lancet Infect. Dis. 2011; 11 (10): 760–768.
13. Konradsen H. B. Validation of serotyping of Streptococcus pneumoniae in Europe. Vaccine. 2005; 23: 1368–1373.
14. Pai R., Gertz R. E., Beall B. Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J. Clin. Microbiol. 2006; 44: 124–131.
15. R.S. Kozlov, A.N. Chagaryan, L.V. Kozlova, A.A. Muravyov. Serological description and antibiotic sensitivity of the pneumococci identified in children under 5 years of age in different regions of the Russian Federation. Clinical microbiology and antimicrobial therapy. 2011; 13(2): 177-187.
16. N.A. Mayanskiy, N.M. Alyabyeva, A.M. Ivanenko, O.A. Ponomarenko, L.K. Katosova, A.V. Lazareva, T.V. Kulichenko, L.S. Namazova-Baranova. Bacterial etiology of acute otitis media in children under 5 years of age: role of Streptococcus pneumoniae. Issues of Pediatric Diagnosis. 2013; 5(1): 5-13.
17. L.G. Boronina, E.V. Samatova. Epidemic peculiarities of the Streptococci pneumoniae identified in children at non-invasive pneumococcal infections and nasopharyngeal carriage. Issues of Pediatric Diagnosis. 2013; 5(1): 22-26.
18. Miernyk K., Debyle C., Harker-Jones M., Hummel K. B., Hennessy T. et al. Serotyping of Streptococcus pneumoniae isolates from nasopharyngeal samples: use of an algorithm combining microbiologic, serologic, and sequential multiplex PCR techniques. J. Clin. Microbiol. 2011; 49: 3209–3214. doi: 10.1128/ JCM.00610-11.
19. Croucher N. J., Harris S. R., Fraser C., Quail M. A., Burton J., van der Linden M., McGee L., von Gottberg A., Song J. H., Ko K. S. et al. Rapid pneumococcal evolution in response to clinical interventions. Science. 2011; 331 (6016): 331–434. doi: 10.1126/science.1198545.
20. Moore M. R., Gertz R. E., Jr, Woodbury R. L., Barkocy-Gallagher G. A., Schaffner W., Lexau C. et al. Population snapshot of emergent Streptococcus pneumoniae serotype 19A in the United States, 2005. J. Infect. Dis. 2008; 197: 1016–1027. doi: 10.1086/528996.
21. Wyres K. L., Lambertsen L. M., Croucher N. J., McGee L., von Gottberg A., Linares J., Jacobs M. R., Kristinsson K. G., Beall B. W., Klugman K. P., Parkhill J., Hakenbeck R., Bentley S. D., Brueggemann A. B. Pneumococcal capsular switching: a historical perspective. J. Infect. Dis. 2013; 207 (3): 439–449.
22. K.O. Mironov, A.E. Platonov, R.S. Kozlov. Identification and serotyping of Russian Streptococcus pneumoniae strains using the PCR-based techniques. Clinical microbiology and antimicrobial therapy. 2011; 13(4): 304-313.
23. Pai R., Limor J., Beall B. Use of pyrosequencing to differentiate Streptococcus pneumoniae serotypes 6A and 6B. J. Clin. Microbiol. 2005; 43(9): 4820–4822.
Review
For citations:
Alyab'eva N.M., Blinova T.A., Ponomarenko O.A., Lazareva A.V., Katosova L.K., Mayanskii N.A. MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION. Current Pediatrics. 2013;12(6):30-34. https://doi.org/10.15690/vsp.v12i6.871
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